In-vitro에서 전기이온영동법을 이용한 알렌드로네이트의 경피약물전달

정지영; 심배선; 조동언; 황인식; Jy-Young Jyoung; Bae Sun Shim; Dong Eon Cho; In Sik Hwang

Polymer(Korea), Vol.33, No.3, 237-242, May, 2009

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In-vitro에서 전기이온영동법을 이용한 알렌드로네이트의 경피약물전달

Iontophoretic Transdermal Delivery of Alendronate in Hairless Mouse Skin

정지영, 심배선^†, 조동언, 황인식

케이엠에이치 중앙연구소

Jy-Young Jyoung, Bae Sun Shim^†, Dong Eon Cho, and In Sik Hwang

R&D Center, KMH Co., Ltd., 799, Kwangyang Dong,Dongan Gu, Anyang, Gyeonggi 431-767, Korea

E-mail:

초록

알렌드로네이트의 생체이용률을 높이고 경구복용시 발생하는 부작용을 해소하고자 경피약물전달시스템에 전기이온영동법, microneedle 등을 적용하여 in-vitro 시험 후 약물전달량을 HPLC-Flu를 이용하여 조사하였다. 전기이온영동시험을 위해 사용된 약물 패취는 UV중합법으로 합성하였으며, 이

This study examined the transdermal delivery of alendronate across hairless mouse skin. The effects of iontophoresis, perforation with a microneedle, and a combination of a microneedle pretreatment and iontophoresis were evaluated in vitro test. Hydrogel patches were polymerized by UV polymerization to supply a hydrogel patch to the iontophoretic transdermal drug delivery system. The alendronate content in the iontophoretic delivery patch was 5.0 mg/cm3. The amounts of alendronate that permeated across the hairless mouse skin when current densities of 0.25 and 0.50 mA/cm2 were supplied to the iontophoretic alendronate patch were 0.80±0.03 and 2.00±0.02 μg, respectively. After pretreatment with a microneedle, the amounts of alendronate that permeated across the hairless mouse skin increased to 70.65±0.37 and 162.23±0.40 μg, respectively. The biocompatibility of the iontophoretic alendronate patch was examined according to the international standardization organization 10993.

Keywords:microneedle;iontophoresis;alendronate;transdermal drug delivery.

[References]

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Glikfeld P, Cullander C, Hinz RS, Guy RH, Pharm. Res., 5, 443 (1988)
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[1] Kanis JA, WHO Study Group, Osteoporos Int., pp 368-381 (1994)

[2] Skorey K, Ly HD, Kelly J, Hammond M, Ramacjamdran C, Huang Z, Gresser MJ, Wang Q, J. Biolo. Chem., 272, 22472 (1997)

[3] Liberman UA, Weiss SR, Broll J, Minne HW, Quan H, Bell NH, Rodriguez-Portales J, Dowms Jr.RW, Dequeker J, Favus M, Seeman E, Recker RR, Capizzi T, Samtora II AC, Lombardi A, Shan RV, Hirsch LJ, Karpf DB, N. Engl. J. Med., 333, 1437 (1995)

[4] Odvina CV, Zerwekh JE, Rao DS, Maalouf N, Gottschalk FA, Pak CYC, J. Clin. Endo. & Meta., 90, 1294 (2005)

[5] Black DM, Thompsom DE, Bauer DC, Ensrud K, Musliner T, Hochberg MC, Nevitt MC, Suryawanshi S, Cummings SR, J. Clin. Endo. & Meta., 85, 4118 (2000)

[6] Orwoll E, Ettinger M, Weiss S, Miller A, Kendler D, Graham J, Adami S, Weber K, Loernc R, Pietschmann P, Vandormael K, Lombardi A, N. Engl. J. Med., 243, 604 (2000)

[7] Bone HG, Hosking D, Devogelaer JP, Tucci JR, Emkey RD, Tonino RP, Rodriguez-Porales JA, Downs RW, Gupta J, Santora AC, Liberman RA, N. Engl. J. Med., 350, 1189 (2004)

[8] Gertz BJ, Holland SD, Kline WF, Matuszewski BK, Freeman A, Hui Q, Lasseter KC, Mucklow JC, Porras AG, Clin. Pharm. Ther., 58, 288 (1995)

[9] Ragsdale AB, Barringer III TA, Anastasio GD, Arch. Fam. Med., 7, 583 (1998)

[10] Delgado-Charro MB, Guy RH, Pharam. Res., 20, 1508 (2003)

[11] Marro D, Guy RH, Delgado-Charro MB, J. Control. Release, 70, 213 (2001)

[12] Tomohira T, Machida Y, Onishi H, Nagai T, Inter. J. Pharm., 155, 231 (1997)

[13] Costello CT, Jeske AH, Phys. Ther., 75, 554 (1995)

[14] Prausnitz MR, Bose VG, Langer R, Weaver JC, Proc. Natl. Acad. Sci. U. S. A., 90, 10504 (1993)

[15] Cross SE, Roberts MS, Current Drug Delivery, 1, 81 (2004)

[16] Ji J, Tay REH, Miao J, Iliescu C, J. Phys.: Conference Series, 34, 1127 (2006)

[17] Wu WM, Todo H, Sugibayashi K, J. Control. Release, 118, 189 (2007)

[18] Rawat S, Vengurlekar S, Rakesh B, Jain S, Spikarti G, Indian J. Pharm. Sci., 70, 5 (2008)

[19] Glikfeld P, Cullander C, Hinz RS, Guy RH, Pharm. Res., 5, 443 (1988)

[20] Taha EA, Youssef NF, Chem. Pharm. Bull., 51, 1444 (2003)

[21] Kuljanin J, Jankovic L, Nedeljkovic J, Prstojevic D, Marinkovic V, J. Pharm. Biomed. Anal., 28, 1215 (2002)

[22] Deeb SKA, Hamdan II, Najjar SMA, Talanta, 64, 695 (2004)

[23] Ptacek P, Klima J, Macek J, J. Chrom. B, 767, 111 (2002)

[24] Yun MH, Woo JS, Kwon KI, Arch. Pharm. Res., 29, 328 (2006)