Lead, copper, and cadmium were adsorbed onto aged calcium alginate beads containing cell-suspension from the waste of beer fermentation broth. Beads prepared by adding 0.6% (w/v) sodium alginate into the cell suspension from the waste of beer fermentation broth and making the cell suspension drop into the 1% (w/v) calcium alginate solution were stored in the 1% (w/v) calcium chloride solution for 1 year. The specific metal uptake of the aged cell-suspension immobilized bead was 312 mg Pb^(2+), 158 mg Cu^(2+), and 112 mg Cd^(2+)/g bead dry weight at pH 7.5 of the metal solution, respectively. The relation between the specific metal uptake by the aged cell-suspension immobilized beads and the equilibrium metal concentration was nonlinearly regressed and well described by the Freundlich isotherm. The specific cadmium uptake capacity of aged cell-suspension immobilized beads was between the specific cadmium uptake capacities of commercial beads Duolite GT-73 and Amberite IRA-400 and higher than those of the fresh Saccharomyces cerevisiae ATCC 834 and Saccharomyces cerevisiae ATCC 24858 immobilized beads.