화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.33, No.1, 7-12, January, 2009
Export Citation
Alginate/PLGA 미립구에 대한 인간디스크 세포 부착 효과
Adhesion of Human Intervertebral Disk Cells on Alginate/PLGA Microspheres
이준희, 장지욱, 소정원, 최진희, 박종학, 안식일, 손영숙1, 민병현2, 강길선†
  • 전북대학교 BK-21 고분자 BIN융합연구팀
  • 1경희대학교 생명과학대학
  • 2아주대학교 세포치료센터
Jun Hee Lee, Ji Wook Jang, Jeong Won So, Jin Hee Choi, Jong Hak Park, Shik il Ahn, Young Suk Son1, Byoung-Hyun Min2, and Gilson Khang†
  • BK21 Polymer BIN Fusion Res. Team, Chonbuk National University, 664-14, Dukjin, Jeonju 561-756, Korea
  • 1College of Life Science, Kyung Hee University, Gyeonggi, Youngin 446-701, Korea
  • 2Cell Therapy Center, School of Medicine, Ajou University, Suwon 442-721, Korea
E-mail:
초록
PLGA미립구는 주사제형과 같은 형태로 조직공학적 응용에 이용할 수 있다. 본 연구에서는 미립구 제조시 에멀젼 형성조건에 대한 영향과 미립구 표면에 세포를 부착시키는 방법에 대하여 연구하였다. BSA를 함유하는 PLGA미립구는 수중유형(O/W)과 수중유중수형(W/O/W) 용매증발법을 이용하여 제조하였다. 미립구의 초기방출효과제어와 PLGA분해의 과정에서 발생되는 지연시간을 개선시키기 위하여 알긴산나트륨을 수상에 용해시켜 사용하였다. 미립구에 부착된 세포의 형태를 전자주사현미경(SEM)을 이용하여 분석하였고 PLGA미립구에 배양된 인간디스크세포의 증식은 MTT분석을 이용하였으며 이를 통하여 PLGA미립구 표면에 세포가 부착되었음을 확인하였다. 본 연구는 BSA가 함유된 알긴산/PLGA미립구를 이용하여 조직공학적 응용이 가능한 주사제형으로서의 가능성을 제안하였다.
PLGA microspheres have been known as an injectable system for tissue engineering. The purpose of this study was to investigate the condition of emulsion formation and cell adhesion on the microsphere surface. BSA-loaded PLGA microsphere was fabricated by oil-in-water (O/W) and water-in-oil-in-water (W/O/W) solvent evaporation method. Sodium alginate was dissolved in water phase to control initial burst release and to improve lag time by PLGA bulk degradation. In addition, the morphology of cells attached on the microspheres was studied using a scanning electron microscopy (SEM). Cellular proliferation behavior of human disc cells cultivated on PLGA microspheres was analyzed using a MTT assay. MTT assay revealed that the cells can attach and proliferate on PLGA microspheres. According to these results, we concluded that BSA-loaded alginate/PLGA microspheres can be used as an injectable system for tissue engineering application.
Keywords:alginate;PLGA;microsphere;cell adhesion;initial burst
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