Several series of porous beads were prepared by suspension polymerization of methyl acrylate and divinyl benzene in the presence of one or two kinds of porogen. The activity of Candida cylindracea lipase (E.C.3.1.1.3) (CCL) adsorbed on these carriers was investigated. Some rules were found for the influence of pore structure such as specific surface area, average pore diameter, and pore volume on the immobilization of CCL. Functional groups attached to these carriers could increase their binding capacity. The optimal conditions for adsorbing CCL were studied. PMA-30-175 and HFPMA-30-175 was chosen for the preparation of immobilized CCL, and the effects of pH, ionic strength, and temperature on the immobilized CCL were compared with those on dissolved CCL.