화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.32, No.6, 516-522, November, 2008
Export Citation
조직공학적 섬유륜재생을 위한 PLGA 지지체 제조시 다공크기의 영향에 관한 연구
The Effect of Pore Sizes on Poly(L-lactide-co-glycolide) Scaffolds for Annulus Fibrosus Tissue Regeneration
소정원, 장지욱, 김순희, 최진희, 이종문, 민병현1, 강길선†
  • 전북대학교 고분자 소재융합연구센터
  • 1아주대학교 세포치료센터
Jeong Won So, Ji Wook Jang, Soon Hee Kim, Jin Hee Choi, John M. Rhee, Byung Hyun Min1, and Gilson Khang†
  • BK-21 Polymer BIN Fusion Research Center, Chonbuk National University, 664-14, Dukjin Dong 1Ga, Dukjin Ku, Jeonju 561-756, Korea
  • 1Cell therapy Center, School of Medicine, Ajou University, Suwon 442-721, Korea
E-mail:
초록
생분해성 고분자인 poly(L-lactide-co-glycolide)(PLGA)를 이용한 조직공학용 다공성 지지체에서의 공극률, 공극의 크기, 공극의 모양 등은 주입된 세포들이 안착하여 증식하는데 있어서 중요한 요건 중 하나이다. 본 연구에서는 섬유륜 세포와 다공크기와의 관계를 파악하고자 다공형성물질인 염화나트륨을 다섯 개의 범위로 분류하여 용매캐스팅/염추출법을 이용한 다양한 다공크기를 갖는 다공성 지지체를 제조하였다. (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium-bromide)(MMT) 분석방법을 이용하여 제조된 지지체에 파종된 섬유륜 세포의 생존율과 증식률을 확인하였으며, in vitro 환경에서의 콜라겐 양과 DNA량을 측정하였다. In vivo 환경에의 세포간의 발생하는 여러 상호작용을 확인하기 위하여 면역결핍 쥐의 피하에 섬유륜 세포가 파종된 지지체를 이식하여 sulfated glycosaminoglycan(sGAG)의 합성정도와 조직학적인 평가를 수행하였다. 결론적으로 180~250 μm 다공크기를 갖는 지지체에서 높은 세포 생존율과 체내에서의 원할한 세포외기질의 형성을 보임으로써 여타의 지지체보다 섬유륜 조직 재생에 적절할 것으로 사료된다.
Biodegradable polymers have been used extensively as scaffolding materials to regenerate new tissues and the ingrowth of tissue have been reported to be dependent directly of the porosity, pore diameter, pore shape, and porous structure of the scaffold. In this study, porous poly(L-lactideco-glycolide)(PLGA) scaffolds with five different pore sizes were fabricated to investigate the effect of pore sizes for AF tissue regeneration. Cellular viability and proliferation were assayed by MTT test. Hydroxyproline/DNA content of AF cells on each scaffold was measured. sGAG analyses were performed at each time point of 2 and 6 weeks. Scaffold seeded AF cells were implanted into the back of athymic nude mouse to observe the difference of formation of disc-like tissue depending on pore size in vivo. We confirmed that scaffold with 180~250 μm pores displayed high cell viability in vitro and produced higher ECM than scaffold with other pore sizes in vivo.
Keywords:tissue engineering;annulus fibrosus cell;PLGA;scaffold;pore size
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