Effects of di-(2-ethylhexyl) phthalate (DEHP) released from laboratory equipments

Dung H. Nguyen; Duc T. M. Nguyen; Eun-Ki Kim

Korean Journal of Chemical Engineering, Vol.25, No.5, 1136-1139, September, 2008

DOI10.1007/s11814-008-0186-z Export Citation

Effects of di-(2-ethylhexyl) phthalate (DEHP) released from laboratory equipments

Dung H. Nguyen, Duc T. M. Nguyen, and Eun-Ki Kim^†

Department of Biological Engineering, Inha University, Incheon 402-751, Korea

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Di-(2-ethylhexyl) phthalate (DEHP) is a plasticizer used in polyvinyl chloride (PVC) manufacturing and is an endocrine disrupter. DEHP was released from laboratory tubing and resins during solvent extraction of natural plants to isolate skin depigmenting compounds. Contamination of DEHP significantly interfered with the purification of depigmenting compounds, since DEHP showed high depigmenting activity (IC50=24 μM) and did not show cell toxicity up to 20 μM. Release of DEHP depended on the composition of tubing materials and solvents used in the extraction process. This result provides practical information for the proper selection of laboratory materials and solvents especially in the extraction and isolation of skin depigmenting compounds for cosmetic ingredients.

Keywords:Di-(2-ethylhexyl) Phthalate;Melanogenesis;Solvent Extraction

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[Related Articles]

[1] Kavlock R, Boekelheide K, Chapin R, Cunningham M, Faustman E, Foster P, Golub M, Henderson R, Hinberg I, Little R, Seed J, Shea K, Tabacova S, Tyl R, Williams P, Zacharewski T, Reprod. Toxicol, 16, 529 (2002)

[2] Tickner JA, Schettler T, Guidotti T, McCally M, Rossi M, Am. J. Ind. Med., 39, 100 (2001)

[3] Fay M, Brattin WJ, Donohue JM, Toxicol. Ind. Health, 15, 707 (1999)

[4] Baue MJ, Herrmann R, Sci. Total Environ., 208, 49 (1997)

[5] Hirosawa N, Yano K, Suzuki Y, Sakamoto Y, Proteomics, 6, 958 (2006)

[6] Takano H, Yanagisawa R, Inoue KI, Ichinose T, Sadakane K, Yoshikawa T, Environ. Health Perspect., 114, 1266 (2006)

[7] Gray E, Wolf C, Lambright C, Mann P, Price M, Cooper R, Ostby J, Toxicol. Ind. Health, 14, 94 (1999)

[8] Li L, Jester W, Orth J, Toxicol. Appl. Pharmacol., 152, 258 (1995)

[9] Ward Jm, Peters JM, Perella CM, Gonzalez FJ, Toxicol. and Pathol., 26, 240 (1998)

[10] Williams DT, J. Agric. Food Chem., 21, 1128 (1973)

[11] Giam CS, Chan HS, Neff GS, Anal. Chem., 47, 2225 (1975)

[12] Riley PA, Pigment Cell Res., 16, 548 (2003)

[13] Petit L, Pierard GE, Int. J. Cosmet. Sci., 25, 169 (2003)

[14] Kadekaro AL, Kavanagh RJ, Wakamatsu K, Ito S, Pipitone MA, Abdel-Malek ZA, Pigment Cell Res., 16, 434 (2003)

[15] Cayce KA, Michael AJM, Feldman SR, Dermatol. Nur., 401, 6 (2004)

[16] Lee HI, Yoo BS, Yoo MA, Byun SY, Korean J. Chem. Eng., 24(4), 655 (2007)

[17] Briganti S, Camera E, Picardo M, Pigment Cell Res., 16, 101 (2003)

[18] Solano F, Briganti S, Picardo M, Ghanem G, Pigment Cell Res., 19, 550 (2006)

[19] Kim DS, Kim SY, Park SH, Choi KY, Kwon SB, Kim MK, Na JI, Youn SW, Park KC, Biol. Pharm. Bull., 28, 2216 (2005)

[20] Tim M, J. Immunol. Methods, 665, 55 (1983)

[21] Cai Q, Mo C, Zeng Q, Li Y, Xiao K, Li H, Xu G, Wang B, Wu Q, Chinese Journal of Applied Ecology, 15, 1455 (2004)

[22] Du Q, Shen L, Xiu L, Jerz G, Winterhalter P, Food Addit. Contam., 23, 552 (2006)