Experimental investigation made previously on microbiological leaching of nickel and cobalt from the laterite nickel ore of Sukinda Valley reveals that the recovery was not very much promising under any favorable conditions. Therefore, in order to improve the efficiency for bioleaching, the homogenized lateritic ore in palletized form is thermally pretreated by roasting at different temperatures. The parameters studied for the bioleaching experiments were the four types of pretreated ore which were roasted at different temperatures, i.e., 300 ℃, 400 ℃, 600 ℃ and 800 ℃, in shake flask by using a mixed mesophilic acidophilic bacterial consortium consisting predominantly of the Acidithiobacillus ferrooxidans strain. It was observed that the pretreated ore at 600 ℃ with 10% (w/v) pulp density showed maximum recovery of nickel and cobalt, i.e., 59.18% (4.556 ppm) and 65.09% (0.546 ppm), using 10% (v/v) (2.5×108 cells/ml) consortium concentration at 1.5 pH, 30 ℃, and 150 rpm after an incubation period of 31 days.