Salvia Miltiorrhiza Bunge로부터 Tanshinone IIA의 용매추출

만효룡; 정용안; 노경호; Xiaolong Wan; Yong An Jung; Kyung Ho Row

Korean Chemical Engineering Research, Vol.46, No.4, 660-664, August, 2008

Export Citation

Salvia Miltiorrhiza Bunge로부터 Tanshinone IIA의 용매추출

Solvent Extraction of Tanshinone IIA from Salvia Miltiorrhiza Bunge

만효룡, 정용안¹, 노경호 ^†

인하대학교 화학공학과 초정밀생물분리기술센터, 402-751 인천시 남구 용현동 253
¹한국기기유화시험연구원 화학분석평가팀, 135-892 서울시 강남구 신사동 587-10

Xiaolong Wan, Yong An Jung¹, and Kyung Ho Row^†

Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong,Nam-Ku, Incheon 402-751, Korea
¹Chemical Analysis & Research Team, Korea Machinery-Meter and Petrochemical Testing & Research Institute, 587-10, Sinsa-Dong, Gangnam-Gu, Seoul 135-892, Korea

E-mail:

초록

본 연구에서는 분석용 HPLC를 사용하여 단삼(Salvia Miltiorrhiza Bunge; SMB)의 추출물로부터 Tanshinone IIA (TIIA)를 분리하였다. 우선 유기용매를 사용하여 추출 및 전처리한 단삼 추출액으로부터 HPLC를 사용하여 TIIA을 분석하였으며, 고순도의 TIIA을 분리하기 위한 최적의 분석조건을 실험적으로 구하였다. 모든 단삼 시료들은 메탄올, 에틸아세테이트, 에탄올 등과 같은 유기용매로 추출하고 비교 분석하였다. 실험결과에 의하면 추출용매로 메탄올을 사용한 경우, 단삼으로부터 TIIA의 추출효율이 가장 우수 하였다. 또한 TIIA를 분석하기 위한 조건으로는 C18 컬럼을 사용 하였고, 이동상은 물과 메탄올을 사용하여 주로 등용매 용리를 적용하였을 때, SMB 1 mg에 2.154 μg TIIA를 분말로 추출하였다.

In this work, analytical HPLC was utilized to obtain Tanshinone IIA (TIIA) from Salvia Miltiorrhiza Bunge (SMB). The optimum operating conditions were experimentally determined to analyze the TIIA in the pretreated extract. SMB was extracted with the various organic solvents of methanol, ethyl acetate, and ethanol, then the extract was analyzed to compare the amount of TIIA. From the results, the methanol showed the best extraction efficiency of TIIAd. The analysis by C18 column was performed. The mobile phase was composed of methanol and water, and the isocratic elution mode was mainly applied. 2.154 μg of TIIA/mg of SMB powder was extracted with methanol.

Keywords:Salvia Miltiorrhiza Bunge;Tanshinone IIA;Extraction;Purification;RP-HPLC

[References]

Han WS, Korean J. Medicinal Crop Sci., 12(3), 179 (2004)
Dean JR, Liu B, Price R, J. Chromatogr. A, 799(1-2), 343 (1998)
Yoon YS, Kim YO, Jeon WK, Park HJ, Sung HJ, J. Ethnopharmacol., 68(1-3), 121 (1999)
Son KH, Park MJ, Lee SH, Park JH, Korean J. Pharmacogn, 30(2), 158 (1999)
Chen J, Wang F, Lee FSC, Wang X, Xie M, Talanta, 69(1), 172 (2006)
Ding M, Ye TX, Zhao GR, Yuan YJ, Guo ZX, International Immunopharmacology, 5(11), 641 (2005)
Ikeshiro Y, Mase I, Tomita Y, Phytochemistry, 28(11), 3139 (1989)
Pan X, Niu G, Liu H, Biochem. Eng. J., 12(1), 71 (2002)
Kim HH, Kim JH, Kwak HBHH, Han SH, Ha H, Lee SW, Woo ER, Lee ZH, Biochemical Pharmacology, 67(9), 1647 (2004)
Gu M, Zhang G, Su Z, Ouyang F, J. Chromatogr. A, 1041(1-2), 239 (2004)
Wan JMF, Sit WH, Lee CL, Fu KHM, Chan DKO, International Immunopharmacology, 6(5), 750 (2006)
Hong SP, Row KH, HWAHAK KONGHAK, 40(4), 488 (2002)
Shi Z, He J, Chang W, Talanta, 64(2), 401 (2004)
Yang Q, Zhang Xl, Li XY, Tang WK, Zhang JX, Fang CX, Zheng CY, Anal. Chim. Acta, 589(2), 231 (2007)
Chang CJ, Chiu KL, Chen YL, Chang CY, Food Chem., 68(1), 109 (2000)
Han SK, Lee KJ, Kim JD, Lee YW, Row KH, Korean Chem. Eng. Res., 42(6), 669 (2004)
Li HB, Chen F, J. Chromatogr. A, 925(1-2), 109 (2001)
Row KH, “Principles and Applications of Liquid Chromatography,” (1999)

[Related Articles]

[1] Han WS, Korean J. Medicinal Crop Sci., 12(3), 179 (2004)

[2] Dean JR, Liu B, Price R, J. Chromatogr. A, 799(1-2), 343 (1998)

[3] Yoon YS, Kim YO, Jeon WK, Park HJ, Sung HJ, J. Ethnopharmacol., 68(1-3), 121 (1999)

[4] Son KH, Park MJ, Lee SH, Park JH, Korean J. Pharmacogn, 30(2), 158 (1999)

[5] Chen J, Wang F, Lee FSC, Wang X, Xie M, Talanta, 69(1), 172 (2006)

[6] Ding M, Ye TX, Zhao GR, Yuan YJ, Guo ZX, International Immunopharmacology, 5(11), 641 (2005)

[7] Ikeshiro Y, Mase I, Tomita Y, Phytochemistry, 28(11), 3139 (1989)

[8] Pan X, Niu G, Liu H, Biochem. Eng. J., 12(1), 71 (2002)

[9] Kim HH, Kim JH, Kwak HBHH, Han SH, Ha H, Lee SW, Woo ER, Lee ZH, Biochemical Pharmacology, 67(9), 1647 (2004)

[10] Gu M, Zhang G, Su Z, Ouyang F, J. Chromatogr. A, 1041(1-2), 239 (2004)

[11] Wan JMF, Sit WH, Lee CL, Fu KHM, Chan DKO, International Immunopharmacology, 6(5), 750 (2006)

[12] Hong SP, Row KH, HWAHAK KONGHAK, 40(4), 488 (2002)

[13] Shi Z, He J, Chang W, Talanta, 64(2), 401 (2004)

[14] Yang Q, Zhang Xl, Li XY, Tang WK, Zhang JX, Fang CX, Zheng CY, Anal. Chim. Acta, 589(2), 231 (2007)

[15] Chang CJ, Chiu KL, Chen YL, Chang CY, Food Chem., 68(1), 109 (2000)

[16] Han SK, Lee KJ, Kim JD, Lee YW, Row KH, Korean Chem. Eng. Res., 42(6), 669 (2004)

[17] Li HB, Chen F, J. Chromatogr. A, 925(1-2), 109 (2001)

[18] Row KH, “Principles and Applications of Liquid Chromatography,” (1999)