An essential step in the development of microfluidic-biochips is represented by the assembly process. Among the thermal bonding processes used for the assembly of such devices the laser transmission welding of polymers offers several advantages, especially when it comes about local deposition of energy and minimum thermal distortion in the joining components. The research presented in this paper proposes a new approach for the laser transmission welding developed for the packaging of disposable polymeric microfluidic-biochips. The new approach based on the use of a fibre laser and a tailored method for the laser energy deposition was tested on the sealing of polymeric biochips made from plexiglas and polypropylene with a covering foil. This method combines the characteristics of the polymer contour welding and quasi-simultaneous welding and allows the achievement of contamination-free, high quality weld seams as narrow as 100 mu m with a high dynamic making it suitable for the high volume production also. (C) 2007 Elsevier B.V. All rights reserved.