Soil carbon contents were measured on a short-rotation woody crop study located on the US Department of Energy's Savannah River Site outside Aiken, SC. This study included fertilization and irrigation treatments on five tree genotypes (sweetgum, loblolly pine, sycamore and two eastern cottonwood clones). Prior to study installation, the previous pine stand was harvested and the remaining slash and stumps were pulverized and incorporated 30cm into the soil. One year after harvest soil carbon levels were consistent with pre-harvest levels but dropped in the third year below pre-harvest levels. Tillage increased soil carbon contents, after three years, as compared with adjacent plots that were not part of the study but where harvested, but not tilled, at the same time. When the soil response to the individual treatments for each genotype was examined, one cottonwood clone (ST66), when irrigated and fertilized, had higher total soil carbon and mineral associated carbon in the upper 30 cm compared with the other tree genotypes. This suggests that root development in ST66 may have been stimulated by the irrigation plus fertilization treatment. Published by Elsevier Ltd.