The effect of medium doses of gamma-irradiation (1-10 kGy) on total phenolic and tannin contents, lipid peroxidation, protein oxidation intensity and soluble protein content of soybean seeds, ( genotype Ana), was investigated. Screening for antioxidant ability was performed using FRAP and DPPH methods. Total phenolic and tannin contents and DPPH scavenger activity were increased, while protein oxidation intensity was decreased by applied doses of gamma-irradiation. Gamma irradiation provoked insignificant changes in lipid peroxidation and soluble protein content, while protein oxidation intensity was significantly decreased when dose of 10 kGy was applied. Presented results implicated that increased antioxidant capacity and protein stability of soybean seeds were increased after application of gamma-irradiation.