In an attempt to obtain biologic control agents for grey mildew of tomato, a total of 628 bacterial strains were isolated from agricultural soil samples in Beijing, China, and screened for in vitro antibiosis toward Botrytis cinerea. Strain P94 exhibited the most obvious antagonistic activity. It P94 had no pathogenicity and was identified as Pseudomonas corrugata by the Biolog identification system combined with 16S rDNA sequence analysis and biochemical and physiologic characteristics. The specific products of polymerase chain reaction with two pairs of specific primers indicated that P94 belonged to P. corrugata genomic group II. Strain P94 inhibited the growth of a number of phytopathogenic fungal and bacterial species and showed inhibition activity to tomato grey mildew by tomato leaf testing in vitro. Strain P94 showed a positive reaction for HCN, protease, phosphatase, and indole acetic acid tests and a negative reaction for siderophore-, chitinase-, and cellulase-production tests. Therefore, the secondary metabolites producing novel P. corrugata strain P94 exhibited an innate potential of biocontrol activities in vitro.