The electrochemical behaviour of N-acetyl-L-cysteine (NAC) has been investigated by linear and cyclic voltarnmetry on gold electrode at room temperature. The results showed two oxidation peaks under acid and neutral conditions and only one in basic medium. For each oxidation, as many electron was exchanged as proton. The influence of both the concentration and the potential scan rate on the peak cur-rents highlighted a diffusion-controlled phenomenon for the first peak and an adsorption-limited reaction rate for the second one. The diffusion coefficient of NAC in solution and the surface concentration of the adsorbed species at pH 3 and 7 were close to 2 x 10(-4) to 2 x 10(-5) cm(2) s(-1) and 6 x 10(-9) to 6 x 10(-10) mol cm(-2), respectively. Film transfer experiments resulted in an irreversible adsorption of NAC on gold electrode, and the formation of a self-assembled monolayer (SAM). (C) 2007 Elsevier Ltd. All rights reserved.