Experimental and numerical studies were conducted to reveal the flow and heat transfer characteristics of liquid laminar flow in microtubes. Both the smooth fused silica and rough stainless steel microtubes were used with the hydraulic diameters of 50-100 Pin and 373-1570 mu m, respectively. For the stainless steel tubes, the corresponding surface relative roughness was 2.4%, 1.4%, 0.95%. The experiment was conducted with deionized water at the Reynolds number from 20 to 2400. The experimental data revealed that the friction factor was well predicted with conventional theory for the smooth fused silica tubes. For the rough stainless steel tubes, the friction factor was higher than the prediction of the conventional theory, and increased as the surface relative roughness increased. The results also confirmed that the conventional friction prediction was valid for water flow through microtube with a relative surface roughness less than about 1.5%. The experimental results of local Nusselt number distribution along the axial direction of the stainless steel tubes do not accord with the conventional results when Reynolds number is low and the relative thickness of the tube wall is high. The numerical study reveals that the large ratio of wall thickness over tube diameter in low Reynolds number region causes significant axial heat conduction in the tube wall, leading to a non-linear distribution of the fluid temperature along the axial direction. The axial heat conduction effect is gradually weakened with the increase of Reynolds number and the decrease of the relative tube wall thickness and thus the local Nusselt number approaches the conventional theory prediction. (c) 2007 Elsevier Ltd. All rights reserved.