The morphological evolution of poly(butylene succinate) (PBS) solution-grown single crystals during annueling was studied using hot-stage atomic force microscopy. Their morphology changed with increasing temperature and annealing time. The annealing behavior and melting temperature were found to be affected by the substate. Morphological changes occured at a much lower temperature on an amorphous carbon film than that on a mica surface. Moreover, the pattern of morphological evolution of the single crystals on a carbon film was different from that on a mica surface. Since the PBS melt had a larger contract angle on the mica surface,these differences in the melting behavior were ascribed to the different interfacial interactions between the chain-folded surface of the single crystal and the substrate. [GRAPHICS] , ranging in thickness from 300 nm to 2 mu m, were successfully formed. [GRAPHICS] ng variety of supramolecular structures and transformation exhibited by polyampholytes when hydrophobic and other non-charge dependent interactions are operative.