Current Applied Physics, Vol.7, No.4, 360-366, 2007
Magnetophoretic concentrating of diamagnetic bio-particles and formation of liquid-crystalline chromatin structures
The packing of chromatin during the self-assembly was modeled with a diamagnetic compression achieved under a gradient magnetic separation (magnetophoresis). Nucleoprotein granules separated from cultivated connective tissue and nuclei of chicken red blood cells formed various chromatin mesophases and chiral supra-molecular structures. The applied magnetic pressure accelerated mesophase formations due to the diamagnetic alignment and the concentrating of nucleosome nanoparticles and aggregates (germs) in gradient magnetic fields. Under various magnetic force parameters (H (.) grad H) similar to 10(5) - 10(11) Oe(2)/cm the different mesophases emerged. Chicken nucleated red blood cells were found to be a convenient model to study the chromatin condensation under influences including electromagnetic factors. (c) 2006 Elsevier B.V. All rights reserved.
Keywords:chromatin self-assembly;chromatin condensation;DNA granules;magnetic separation;nucleosome core particles