We present a method for the speciation of chromium by capillary electrophoresis. Cr(III) was complexed with diethylenetriaminepentaacetic acid (DTPA) to form a negatively charged complex. Using 20 mm phosphate buffer of pH 8 containing 0.5 mm tetradecyltrimethylammonium hydroxide (TTAOH) at a separation voltage of -15 kV, both forms of chromium CrDTPA(2-) and CrO42- were separated in less than 6 min. Direct UV detection at 214 nm was used. The effect of the presence of interfering ions was investigated. The application of the developed method to speciation of chromium in tanning liquor is demonstrated. The obtained results have shown a good correlation with those of flame atomic absorbance spectrometry (FAAS), inductively coupled plasma-mass spectrometry (ICP-MS) and UV/VIS spectrophotometry.