Generation of minimal protein identifiers of proteins from two-dimensional gels and recombinant proteins

Schmidt F; Lueking A; Nordhoff E; Gobom J; Klose J; Seitz H; Egelhofer V; Eickhoff H; Lehrach H; Cahill DJ

Electrophoresis, Vol.23, No.4, 621-625, 2002

DOI10.1002/1522-2683(200202)23:4<621::AID-ELPS621>3.0.CO;2-J Export Citation

Generation of minimal protein identifiers of proteins from two-dimensional gels and recombinant proteins

Schmidt F, Lueking A, Nordhoff E, Gobom J, Klose J, Seitz H, Egelhofer V, Eickhoff H, Lehrach H, Cahill DJ

We describe the technical feasibility and methodology to characterize a protein by a minimal set of structural information generated by matrix assisted laser desorption/ ionization (MALDI)-mass spectrometry, termed a "minimal protein Identifier" (MPI). MPIs can be determined for proteins from two-dimensional gels and recombinant proteins and can be used to compare and identify proteins from these sources.

Keywords:matrix assisted laser desorption;ionization-time of flight-mass spectrometry minimal protein identifier;protein arrays;proteomics;two-dimensional gel electrophoresis