Bio-diesels play an important role in meeting future fuel requirements in view of their nature (less toxic), and have an edge over conventional diesel as they are obtained from renewable sources. Bio-diesel blends up to 20% in diesel are being used at present. In this article, the determination of bio-diesel in diesel both by conventional and spectroscopic methods is described. These methods are simple, fast and reliable and can be used to determine bio-diesel in a wide range of concentrations of up to 25% blends.