Liposomes of dipalmitoylphosphatidylcholine containing a photochromic lipid "Bis-Azo PC" release entrapped solutes on exposure to UV light. We have now demonstrated that on addition of cholesterol (up to 25 mol%) to the liposomal membrane the liposomes also release their contents in response to visible light in the region of 470 nm, to which liposomes lacking steroid are insensitive. In a mixed population of liposomes prepared with and without cholesterol, this enables wavelength-dependent release of entrapped solutes on sequential exposure to visible and UV light. Furthermore, the cholesterol-containing liposomes allow stepped partial release of entrapped solute following multiple periods of short visible illumination. It is suggested that the cholesterol-containing liposomes may be potentially useful for drug delivery and for L'Caging" of reagents.