Biochemical and Biophysical Research Communications, Vol.360, No.3, 520-524, 2007
beta-amyloid deposition and prion infection in adult primary brain cell long-term culture model
We report an in vitro model of the adult central nervous system produced by culturing primary brain cells isolated from adult mice for periods longer than 4 months. We applied this novel cell culture method to model progressive neurodegencrative diseases. After long-term culture of adult primary brain cells prepared from Alzheimer's disease and prion disease mouse models, we observed P-amyloid deposition and prion infection in primary cell cultures in vitro. (C) 2007 Elsevier Inc. All rights reserved.