The precursor of amphibian tachykinin has not been found although more than 30 tachykinins have been isolated from amphibians since 1964. In this report, two tachykinin-like peptides are identified from the skin secretions of the frog, Odorrana grahami. Their amino acid sequences are DDTEDLANKFIGLM-NH2 (named tachykinin OGl) and DDASDRAKKFYGLM-NH2 that is the same with ranamargarin found in Rana margaretae, respectively, with a conserved FXGLM-NH2 C-terminal consensus motif. By cDNA cloning, their precursors were screened from the skin cDNA library of O. grahami. The precursors are composed of 61 amino acid (aa) residues including a signal peptide followed by an acidic spacer peptide and one copy of mature tachykinin-like peptide. Their overall structure is different from structures of other tachykinin precursors such as human protachykinin l precursor containing 143 aa including one copy of substance P (SP) and neurokinin A (NKA), and ascidian tachykinin l precursor containing 164 aa including two copies of tachykinin-like peptides. The current results demonstrate that the biosynthesis mode of tachykinins in amphibians is different from other animals. (c) 2006 Elsevier Inc. All rights reserved.