The gliding bacterium Mycoplasma mobile adheres to plastic surfaces and mows around rigorously. However, it has not been possible to control the direction of movements on plain Surfaces. Here we report that oil patterned lithographic substrates, M. mobile cells are unable to climb tall walls and move along the bottom edge of the walls. This property, to move persistently along walls enabled us to design patterns that control direction of movements, resulting ill uni-directional circling or one-way gating between two areas. Furthermore, cells loaded with streptavidin beads following biotinylation of surface proteins moved at normal speeds. These bacteria could be useful as living microtransporters. carrying cargo around within micrometer-scale spaces. © 2005 Elsevier Inc. All rights reserved.