Reaction Kinetics and Catalysis Letters, Vol.83, No.1, 79-83, 2004
Leaching studies over immobilized alpha-amylase. Importance of the nature of enzyme attachment
The enzyme alpha-amylase was immobilized on montmorillonite K-10 via adsorption and covalent binding. These two modes of attachment play a vital role in the leaching of enzyme. Covalent bonding retains most of the enzyme even at high loadings. The significance of temperature has also been established. Higher temperatures lead to severe leaching in case of adsorbed enzyme. Covalent linkage, being of higher strength, does not yield to enzyme leaching even at high temperatures.