A novel fluorometric saccharide detection strategy has been established by utilizing saccharide-induced conformational changes in copolymers containing boronic acid and fluorescent units. The polymer chain changes its conformation due to an alteration of the charge state induced by binding with saccharides. The conformational changes are conveniently detected by monitoring excimer to monomer intensity ratio in the fluorescence spectra. Thus, the present strategy would be a promising way for the creation of highly selective and sensitive saccharide-sensing systems.