Quantification of cell adhesion interactions by AFM: effects of LPS/PMA on the adhesion Of C-6 glioma cell to collagen type I

Kim H; Arakawa H; Osada T; Ikai A

Applied Surface Science, Vol.188, No.3-4, 493-498, 2002

DOI10.1016/S0169-4332(01)00978-3 Export Citation

Quantification of cell adhesion interactions by AFM: effects of LPS/PMA on the adhesion Of C-6 glioma cell to collagen type I

Kim H, Arakawa H, Osada T, Ikai A

Time-course effects of lipopolysaccharide (LIPS) with phorbol-12-myristate-13-acetate (PMA) were quantitatively examined using an atomic force microscope (AFM) cantilever with a type I collagen-coated spherical bead as a probe. LPS/PMA affects the expression level of fibronectin (FN), increasing the adhesion strength for collagen type I about 1.7 times. After 4 days, the adhesion strength for collagen increased 1.6 times. Our method is suitable for quantitative studies of cell adhesion phenomena on the single cell level. (C) 2002 Elsevier Science B.V. All rights reserved.

Keywords:force microscope;fibronectin;collagen type I;microbead;separation work;LPS;PMA;C-6 glioma cell