The search for new materials for chromatographic separation of proteins is a major endeavor nowadays. In order to obtain better chromatographic separation media, poli(glycidyl methacrylate co-ethylen dimethacrylate) matrices were synthesized, milled, classified, functionalized with different reagents and then comparatively tested in batch experiments for protein retention. The Methacrylate,,Dimethaerylate resin was treated in different sequences of experiments with Urea, Iminodiacetic acid, N-N dimethyl formamide and ammonia, in order to determine whether functionalization provides sufficient amino groups to capacitate them for protein retention. Fixed bed experiments with particulate resin were also carried out. Previous characterization confirms that the polymeric matrix is macroporous and exhibits a unimodal pore size distribution. The resin also presents a good thermal stability. (C) 2002 Elsevier Science B.V. All rights reserved.