This paper presents the development of a method for the isolation and refining of microcrystalline wax from press mud waste of the sugar industry. Physico-chemical and XRD analyses were made for characterization of isolated microwax. The yield of hard wax was found to be from 3.50 to 4.10%. The colour of the microwax was light cream and it had a nominal acid value and saponification number. The degree of crystallinity (%) of the wax was found to be 31% for the refined variety and 34% for the doubly refined variety. The method enables high grade refined sugar cane microwax to be obtained.