A general rate model for liquid chromatography which considers nonlinear isotherms and various mass transfer effects, including axial dispersion, interfacial film mass transfer and intraparticle diffusion is applied to the modeling and scale-up of reversed-phase liquid chromatography. The model is solved with a FORTRAN program which is run on a personal computer. With a few simple experiments using a small analytical column, the binding characteristics and the porosity of the packing particles are determined. Mass transfer parameters are evaluated using existing correlations in the literature without any experimentation. Human growth hormone and a recombinant human growth hormone analog (hGHG120R) were used as protein samples in experiments. Gradient elution profiles of a preparative column are predicted without a posteriori data.