A novel sensing system for glucose-specific detection has been established based on a combination of a boronic acid and enzymes. To overcome the inherently low biding affinity of glucose toward boronic acids, glucose is converted into gluconic acid by the enzymatic reaction using glucose oxidase (GOx), and then complexed with a fluorescent boronic acid through the ce-hydroxycarboxylate moiety. According to the present strategy, glucose concentration is exclusively determined among other saccharides in aqueous solutions.