A two-step reduction route in polyol process for producing copper nano-particles with satisfied antioxidation property is described here. This process involves two-step reduction of copper salts in polyol solution with the surfactant protection under atmospheric conditions at a temperature below 353 K. Well-dispersed nano-copper particles with ca. 80 nm in diameter were obtained from the diethylene glycol (DEG) solution containing 0.2 M Cu2+ ion, 0.3 mM polyvinylpyrrolidone (PVP) as a ligand agent at 330K, glucose as first reductant, and ascorbic acid as second reductant.