Covalent immobilization of metal-binding motifs of enzymes on quartz surface. [Ni(CYS-X-2-Cys)(2)](2-) of hydrogenases

Sakaniwa D; Ohe T; Misumi T; Monjushiro H; Onoda A; Yamamura T

Chemistry Letters, Vol.34, No.12, 1634-1635, 2005

DOI10.1246/cl.2005.1634 Export Citation

Covalent immobilization of metal-binding motifs of enzymes on quartz surface. [Ni(CYS-X-2-Cys)(2)](2-) of hydrogenases

Sakaniwa D, Ohe T, Misumi T, Monjushiro H, Onoda A, Yamamura T

The peptide ligand, Cys-Pro-Leu-Cys, was covalently immobilized on the surface of quartz via (3-aminopropyl) triethoxysilane (APTES) by the general peptide-synthesis method; then, Ni2+ was introduced into this surface to construct the [Ni(S-CYS)(4)](2-) unit contained in the active site of hydrogenases.