Chemistry Letters, Vol.34, No.7, 940-941, 2005
Selective detection of the solid-state NMR signals from the bacteriochlorophyll a dimers in a reconstituted light-harvesting 1 complex
High resolution solid-state C-13 NMR spectra have been obtained for bacteriochlorophyll (BChl) a in a light-harvesting (LH) 1 complex utilizing a reconstitution method. The LH I complexes were reconstituted with C-13-enriched BChl a and the apo-LHI polypeptide from Rhodospirillum rubrum. Measurement of the 2-dimentional C-13-C-13 dipole correlation NMR spectra enabled the selective assignment of the 13 C resonance from the BChl a molecules in the reconstituted LH1.