Application of Bis(terpyridine)ruthenium(II) to N-terminal amino acid sequencing

Okamura T; Iwamura T; Ito A; Kaneko M; Yamaguchi M; Yamamoto H; Ueyama N; Kuyama H; Ando E; Norioka S; Nakazawa T; Masui R; Kuramitsu S

Chemistry Letters, Vol.34, No.3, 332-333, 2005

DOI10.1246/cl.2005.332 Export Citation

Application of Bis(terpyridine)ruthenium(II) to N-terminal amino acid sequencing

Okamura T, Iwamura T, Ito A, Kaneko M, Yamaguchi M, Yamamoto H, Ueyama N, Kuyama H, Ando E, Norioka S, Nakazawa T, Masui R, Kuramitsu S

A novel N-terminal-labeling reagent [(tpy)Ru-II(tpyCONS u)](2+) (=< Ru >-COONSu) provides an efficient and effective N-terminal sequencing of peptides and proteins in MALDI-TOF-MS/MS (PSD) analysis, which shows a(n) fragments predominantly without any C-terminal-fragment ions.