Chemistry Letters, Vol.34, No.1, 110-111, 2005
Lyophilization of lipase together with ionic compounds generates highly enantioselective and solvent-sensitive lipase in organic solvents
The lyophilization of lipase from aqueous enzyme solution containing ionic compounds such as disodium hydrogenphosphate resulted in a dramatic improvement of its enantioselectivity for esterification in organic solvents, compared with the ionic compound-free lipase. The enantioselectivity of the ionic compound-coated lipase was found to be fairly sensitive to the change of the solvents.