Direct detection of horseradish peroxidase as a marker molecule encapsulated in liposomes via use of fluorescein chemiluminescence

Kamidate T; Ishida Y; Tani H; Ishida A

Chemistry Letters, Vol.32, No.4, 402-403, 2003

DOI10.1246/cl.2003.402 Export Citation

Direct detection of horseradish peroxidase as a marker molecule encapsulated in liposomes via use of fluorescein chemiluminescence

Kamidate T, Ishida Y, Tani H, Ishida A

A method for direct detection of horseradish peroxidase (HRP) as a marker molecule trapped in liposomes by the use of HRP-catalyzed fluorescein chemiluminescence (CL) with hydrogen peroxide has been developed. Maximum CL emission in the direct detection of HRP in liposomes increased by a factor of 13 times compared with that in the detection of HRP dissolved in lipid-free buffer solution.