New fluorescent ionophores containing I-pyrenyl aromatic amides based on oligoethylene oxide (1 and 2) have been synthesized and its photochemical behaviors have been studied. In the absence of metal ion, 1-pyrenylbenzamide moiety showed almost no fluorescence emission. However, complexation with Ca2+ induced a large enhancement effect on the fluorescence intensity of 1 and 2 from the pyrene ring. This behavior can be explained by in terms of the similar twisted intramolecular charge-transfer relaxation mechanism.