Chemistry Letters, Vol.29, No.1, 76-77, 2000
Non-contact measurement of adsorbed cytochrome c with optical waveguide spectrometry; The effect of distance between waveguide and protein on the spectral sensitivity
Optical waveguide spectrometry with non-contact conditions was performed with the aim of widening the choice of substrate material. The visible absorption spectrum of cytochrome c (cyt.c) adsorbed on a quartz glass or gold surface was detected with the evanescent wave from a co-faced waveguide. A clear correlation was observed between absorbance of cyt.c and the gap between the waveguide surface and the protein.