Determination of the electrostatic potential difference between DNA and the solution containing it: A kinetic approach

Carrasco M; Coca R; Cruz I; Daza S; Espina M; Garcia-Fernandez E; Guerra FJ; Leon R; Marchena MJ; Perez I; Puente M; Suarez E; Valencia I; Villalba I; Jimenez R

Chemical Physics Letters, Vol.441, No.1-3, 148-151, 2007

DOI10.1016/j.cplett.2007.04.090 Export Citation

Determination of the electrostatic potential difference between DNA and the solution containing it: A kinetic approach

Carrasco M, Coca R, Cruz I, Daza S, Espina M, Garcia-Fernandez E, Guerra FJ, Leon R, Marchena MJ, Perez I, Puente M, Suarez E, Valencia I, Villalba I, Jimenez R

A kinetic approach to determine the electrostatic potential difference in soft interfaces, when classical methods fail, is presented. The method is based on electrostatic and non-electrostatic separation of the binding constant of one reactant to one of the phases. Here the method is applied to the DNA/water interface. Reliable results are obtained using as probes the reactions [Ru(NH3),pz] (3+) + S2O82- and [Co(NH3)(5)pz](3+) + [Fe(CN)(6)](4-). (C) 2007 Elsevier B.V. All rights reserved.