A formation of supramolecular host-guest inclusion surface layer is reported. A nanostructure of this layer consists of an inclusion complex, where a guest molecule - coumarin-6 (3-(2-benzothiazolyl)-7-(diethylamino) coumarin, C20H18N2O2S) is placed into a 'bucket' of a host molecule - thiolated cyclodextrin (6-monodeoxy-6-monothio-beta-cyclodextrin, C42H70O34S) which is attached on Au surface. The inclusion and the layer formation are determined by using time-resolved fluorescence anisotropy, second-harmonic generation (SHG), and fluorescence in combination with laser-induced thermal desorption from the surface. (C) 2003 Elsevier Science B.V. All rights reserved.