Coenzyme Q(10) (CoQ(10)) is incorporated in nanoliposomes composed of egg yolk phospholipid, cholesterol, and Tween 80. Atomic force microscopy, performed to characterize vesicle surface topology, shows some visible influence of CoQ(10) on the nanoliposomal structure. CoQ(10) incorporation can suppress the increase of the z-average diameter of nanoliposomes during storage for 8 months at 4 degrees C. The liposomal lipid peroxidation caused by Fe(III)/ascorbate is also significantly inhibited. Perturbation of acyl chain motion of lipids due to the presence of CoQ(10) in the bilayer is examined by fluorescence probe diphenyl-hexatriene and Raman spectroscopy. Fluorescence probe studies indicate that CoQ(10) incorporation results in the microviscosity increase of nanoliposomes. The steric structure of nanoliposomes reflected by Raman spectroscopy changes obviously and shows CoQ(10) content dependency. The order parameters for the lateral interaction between chains increase. The trans conformation decrease and the gauche conformation increase as the weight contents of CoQ(10) incorporation are at 1%, 5%, 10%, and 32.5%. However, the order parameters for the longitudinal interaction in chains was higher than that of pure nanoliposomes as the weight content of CoQ(10) is at 25%. Results suggest that CoQ(10) might intercalate between lipid molecules and perturb the bilayer structure.