Preparation of Collagen/Poly(L-lactic acid) Composite Material for Wound Dressing

Jung Soo Lee; Jae Kyung Kim; Yoon Ho Chang; So Ra Park

Macromolecular Research, Vol.15, No.3, 205-210, April, 2007

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Preparation of Collagen/Poly(L-lactic acid) Composite Material for Wound Dressing

Jung Soo Lee, Jae Kyung Kim, Yoon Ho Chang^†, and So Ra Park¹

Department of Chemical Engineering, Inha University, Incheon 402-751, Korea
¹Department of Physiology, College of Medicine, Inha University, Incheon 402-751, Korea

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Collagen is the major structural protein of connective tissues. It can be used as a prosthetic biomaterial applicable to artificial skin, tendon, ligaments, and collagen implants. The objective of this study is to investigate the possibility of realizing wound dressing medical products by the synthesis of composite materials with collagen and a biodegradable polymer, PLLA, via a surface modification process. Type I collagen was obtained from pig skin by a separation process. The structural characteristics of the extracted collagen were confirmed by SDS-polyacrylamide (PAcr) gel electrophoresis (PAGE) and FTIR. Also, PLLA-g-PAcr was synthesized by the radical polymerization of acrylamide initiated by AIBN in the presence of PLLA. The surface of PLLA was modified by the presence of the acrylamide residues. The structural characteristics of the copolymer were analyzed by FTIR, 1H-NMR and contact angle measurements. The water uptake and WVTR of the collagen/PLLA-g-PAcr composite tended to increase with increasing collagen concentration and with decreasing EDC concentration.

Keywords:collagen;PLLA;acrylamide;wound dressing;biomaterial

[References]

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Browder IW, Litwin MS, Am. Surg., 52, 492 (1986)
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Jung KJ, Ahn KD, Han DK, Ahn DJ, Macromol. Res., 13(5), 446 (2005)
Khang G, Rhee JM, Shin P, Kim IY, Lee B, Lee SJ, Lee YM, Lee HB, Lee I, Macromol. Res., 10(3), 158 (2002)
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Kim KW, Woo SI, Macromol. Chem. Phys., 203, 2245 (2002)
Otera J, Kawada K, Yano T, Chem. Lett., 225 (1996)
Avella M, Errico ME, Immirzi B, Malinconico M, Angew. Makromol. Chem., 246, 49 (1997)
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Nagai T, Ogawa T, Nakamura T, Ito T, Nakagawa H, Fujiki K, Nakao M, Yano T, J. Sci. Food Agr., 79, 855 (1999)
Burjandze TV, Biopolymers, 18, 931 (1979)
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[Referenced By]

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[1] Junqueira LC, Carnerio J, Basic Histology, 4th ed., Lange Medical Publication, 1983, pp. 89-119

[2] Fonseca MJ, Alsina MA, Reig F, Biochim. Biophys. Acta, 1279, 259 (1996)

[3] Maeda M, Tani S, Sano A, Fujioka K, J. Control. Release, 62, 313 (1999)

[4] Kaufman HE, Steinemann TL, Lehman E, Thompson HW, Varnell ED, Gerhardt BM, J. Ocul. Pharmacol., 10, 17 (1994)

[5] Rao KP, J. Biomater. Sci., 7, 623 (1995)

[6] Thacharodi D, Rao KP, Biomaterials, 17, 1307 (1996)

[7] Rossler B, Kreuter J, Scherer D, J. Microencapsul., 12, 49 (1995)

[8] Miller JM, Zoll DR, Brown EO, Arch. Surg., 88, 167 (1964)

[9] Cameron WJ, Obstet. Gynecol., 51, 118 (1978)

[10] Browder IW, Litwin MS, Am. Surg., 52, 492 (1986)

[11] Kemp PD, Grant M, Methods in Molecular Biology, 139, 287 (2000)

[12] Chvapil M, Speer DP, Holubec H, Chvapil TA, King DH, J. Biomed. Mater. Res., 27, 313 (1993)

[13] Auger FA, Rouabhia M, Goulet F, Berthod F, Moulin V, Germain L, Med. Biol. Eng. Comput., 36, 801 (1998)

[14] Jung KJ, Ahn KD, Han DK, Ahn DJ, Macromol. Res., 13(5), 446 (2005)

[15] Khang G, Rhee JM, Shin P, Kim IY, Lee B, Lee SJ, Lee YM, Lee HB, Lee I, Macromol. Res., 10(3), 158 (2002)

[16] Shyamroy S, Garnaik B, Sivaram S, J. Polym. Sci. A: Polym. Chem., 43(10), 2164 (2005)

[17] Kim KW, Woo SI, Macromol. Chem. Phys., 203, 2245 (2002)

[18] Otera J, Kawada K, Yano T, Chem. Lett., 225 (1996)

[19] Avella M, Errico ME, Immirzi B, Malinconico M, Angew. Makromol. Chem., 246, 49 (1997)

[20] Wong WS, Food Chem., 36, 237 (1990)

[21] Nagai T, Ogawa T, Nakamura T, Ito T, Nakagawa H, Fujiki K, Nakao M, Yano T, J. Sci. Food Agr., 79, 855 (1999)

[22] Burjandze TV, Biopolymers, 18, 931 (1979)

[23] Ma Z, Gao C, Shen J, J. Biomater. Sci.-Polym. Ed., 14, 13 (2003)

[24] Calandrelli L, De Rosa G, Errico ME, La Rotonda MI, Laurienzo P, Malinconico M, Oliva A, Quaglia F, J. Biomed. Mater. Res., 62, 244 (2002)