Clostridium beijerinckii Donker 1926을 이용한 혐기성 소화공정에서 체류시간 변화에 의한 수소 생산과 동력학적 특성

정태영; 차기철; 최석순; Tae-Young Jeong; Gi-Cheol Cha; Suk Soon Choi

Journal of the Korean Industrial and Engineering Chemistry, Vol.18, No.2, 162-167, April, 2007

Effect of Hydraulic Retention Time (HRT) on the Hydrogen Production and Its Dynamic Characteristics in the Anaerobic Digestion Process Using Clostridium beijerinckii Donker 1926

정태영 , 차기철, 최석순 ^{1, †}

연세대학교 환경공학부
¹세명대학교 바이오환경공학과

Tae-Young Jeong , Gi-Cheol Cha, and Suk Soon Choi^{1, †}

Division of Environmental Engineering, Yonsei University, Wonju 220-710, Korea
¹Department of Biological and Environmental Engineering, Semyung University, Jecheon 390-711, Korea

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초록

본 연구에서는 Clostridium beijerinckii Donker 1926을 이용한 연속식 혐기성 소화공정에서 수소 생산과 동력학적 특성을 고찰하였다. 기질은 glucose를 사용하였고, 0.5, 0.25, 0.125일의 체류시간 (hydraulic retention time, HRT)에서 실험이 이루어졌으며, 모든 HRT의 조건에서 탄산화물은 99% 이상의 제거효율를 나타내었다. 체류시간이 짧을수록, COD 제거율은 낮은 반면에, 전체 가스 중에서 수소 가스 함량과 수소 발생량이 높게 나타났다. 또한, 정상상태에서, 증식 수율과 수소가스 생성 수율은 각각 0.27 g-VSS/g- glucose, 0.26 L/g-glucose로 나타났다. 본 실험에 사용된 균주를 glucose와 같이 당 성분이 함유된 폐수처리에 적용하면 수소를 생산할 수 있으며, 이러한 실험 결과들을 잘 활용하면 대체에너지로서, 실제적인 수소가스 생산 시스템에 적용할 수 있을 것이다.

Hydrogen production and its dynamics were investigated in the continuous anaerobic digestion process using Clostridium beijerinckii Donker 1926. In this work, glucose was used as a substrate and hydraulic retention times (HRT) were 0.5, 0.25 or 0.125 day. The removal efficiency of carbohydrate was over 99% under all of HRT conditions. As HRT was shorter, COD removal efficiency became lower while hydrogen content in the total gas and hydrogen production rate became higher. The cell growth yield and hydrogen production yield were 0.27 g-VSS/g-glucose and 0.26 L/g-glucose, respectively, at the steady state. It is expected that the microorganism is able to produce hydrogen when used in the wastewater treatment containing carbohydrate such as glucose. Also, the results in this study could be applied to the actual hydrogen gas production, a promising alternative energy.

Keywords:hydrogen production;hydraulic retention time (HRT);glucose;cell growth yield;hydrogen production yield

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[Related Articles]

[1] Jeffries TW, Omstead DR, Cardenas RR, Gregor HP, Biotech. Bioeng. Symp., 9, 37 (1979)

[2] Breue AM, Andel JG, Appl. Microbiol. Biotechnol., 20, 40 (1984)

[3] Fumiaki T, Chang JD, Mizukami N, Tatsuo ST, Katsushige H, Can. J. Microbiol., 39, 726 (1993)

[4] Yokoi H, Ohkawa T, Hirosse J, Hayashi S, Takasaki Y, J. Ferment. Bioeng., 80, 571 (1995)

[5] Gibson AM, Bratchell N, Roberts TA, J. Appl. Bacteriol., 62, 479 (1987)

[6] Taguchi F, Chang JD, Takiguchi S, Morimoto M, J. Ferment. Bioeng., 73, 244 (1992)

[7] Perego P, Fabiano B, Ponzano GP, Palazzi E, Bioprocess Eng., 19, 205 (1998)

[8] Lay JJ, Li YY, Noike T, J. Environ. Eng.-ASCE, 124, 730 (1998)

[9] APHA, Standard Methods for the Examination of Water and Wastewater, 17th ed., American Public Health Association, Washington, DC (1989)

[10] Vavilin VA, Rytov SV, Lokshina LY, Bioresour. Technol., 56(2-3), 229 (1996)

[11] Naoaki K, Akiko M, Koichi K, Water Sci. Technol., 36, 41 (1997)

[12] Ghosh S, Pohland FG, J. Water Poll. Control Fed., 46, 748 (1974)

[13] McCarty PL, Adv. Chem. Ser., 105 (1971)

[14] Monod J, Ann. Rev. Microbiol., 3, 371 (1949)

[15] Zwietering MH, Jongenburger I, Rombouts FM, Riet KV, Appl. Environ. Microbiol., 56, 1875 (1990)

[16] Lehninger AL, Biochemistry, Worth Publishers Inc., New York (1970)