EGCG가 포집된 Ethosome의 제조와 특성조사

곽효정; 진병석; Hyo Jung Gwak; Byung Suk Jin

Journal of the Korean Industrial and Engineering Chemistry, Vol.18, No.2, 130-135, April, 2007

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EGCG가 포집된 Ethosome의 제조와 특성조사

Preparation and Characterization of EGCG Entrapped Ethosome

곽효정, 진병석 ^†

동덕여자대학교 응용화학과

Hyo Jung Gwak, and Byung Suk Jin^†

Department of Applied Chemistry, Dongduk Women’s University, Seoul 136-714, Korea

E-mail:

초록

EGCG의 안정성을 향상시키기 위해 ethosome에 포집을 시도하였다. 물에 에탄올을 첨가해서 EGCG의 용해도를 높임으로써 ethosome에 적정량의 EGCG이 포집될 수 있도록 하였다. EGCG 수용액의 농도와 ethosome 막을 구성하는 지질의 조성은 ethosome 입자크기와 포집효율에 상당한 영향을 미치는 것을 확인하고 ethosome의 액정상 형성은 편광 현미경을 사용하여 관측하였다. EGCG가 수용액 상태로 있을 때와 ethosome에 포집되어 있을 때, UV 또는 고온의 상태 에서의 안정성을 비교한 결과 ethosome에 포집된 EGCG의 안정화 효과를 확인하였다. Ethosome에 토코페롤의 첨가는 UV에 의한 EGCG의 분해를 지연시켰다.

Entrapment of (-)-epigallocatechin gallate (EGCG) into ethosome was carried out for improving its stability against decomposition. Solubility of EGCG was increased by the addition of ethanol into water, which enable ethosome to entrap appropriate amount of EGCG. It was observed that the EGCG solution concentration and constituent lipid composition had a considerable effect on the particle size and entrapment efficiency of ethosomes. The formation of liquid crystalline phase in ethosome was investigated by polarized optical microscopy. By comparing the stability of EGCG in solution and in ethosome exposed to UV or high temperature, we evaluated the EGCG stabilization effect through its entrapment in ethosome. Incorporation of tocopherol into ethosome retarded the decomposition of EGCG under UV.

Keywords:ethosome;EGCG;stability;liquid crystalline phase;entrapment efficiency

[References]

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Yang SC, Maliakal P, Meng X, Annu. Rev. of Pharmacol. Toxicol., 42, 25 (2002)
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[1] Fletcher K, Pharm. Science & Technol. Today, 1, 49 (1998)

[2] Kirjavainen M, Urtti A, Koskela RV, Kiesvaara J, Monkkonen J, Eur. J. Pharm. Sci., 7, 279 (1999)

[3] Egbaria K, Ramachandran C, Weiner N, Skin Pharmacol. Phys., 4, 21 (1991)

[4] Knepp VM, Hinz RS, Szoka FC, Guy RH, J. Control. Release, 5, 211 (1988)

[5] Knepp VM, Szoka FC, Guy RH, J. Control. Release, 12, 25 (1990)

[6] Planas ME, Gonzalez P, Rodriquez L, Sanchez S, Cevc G, Anesth. Analg., 75, 615 (1992)

[7] Cevc G, Blume G, Biochim. Biophys. Acta, 1104, 226 (1992)

[8] Cevc G, Schatzlein A, Blume G, J. Control. Release, 36, 3 (1995)

[9] Cevc G, Blume G, Schatzlein A, Gebauer D, Paul A, Adv. Drug Deliv. Rev., 18, 349 (1996)

[10] Cevc G, Blume G, Schatzlein A, J. Control. Release, 45, 211 (1997)

[11] Paul A, Cevc G, Bachhawat BK, Eur. J. Immunol., 25, 3521 (1995)

[12] Trotta M, Peira E, Debernardi F, Gallarate M, Int. J. Pharm., 241, 319 (2002)

[13] Trotta M, Peira E, Carlotti ME, Gallarate M, Int. J. Pharm., 270, 119 (2004)

[14] Touitou E, Dayan N, Bergelson L, Godin B, Eliaz M, J. Control. Release, 65, 403 (2000)

[15] Touitou E, Godin B, Weiss C, Drug Dev. Res., 50, 406 (2000)

[16] Touitou E, Meidan VM, Horwitz E, J. Control. Release, 56, 7 (1998)

[17] Perrett S, Golding M, Williams WP, J. Pharm. Pharmacol., 43, 154 (1991)

[18] Kirjavainen M, Urtti A, Valjakka-Koskela R, Kiesvaara J, Monkkonen J, Eur. J. Pharm. Sci., 7, 279 (1999)

[19] Jin BS, Han YM, Lee KH, Choi MJ, J. Korean Ind. Eng. Chem., 15(2), 205 (2004)

[20] Jin BS, Lee SM, Lee KH, J. Korean Ind. Eng. Chem., 17(2), 138 (2006)

[21] Fujiki HJ, J. Cancer Res. Clin. Oncol., 125, 589 (1999)

[22] Balentine DA, Wiseman SA, Bouwens LC, Crit. Rev. Food Sci. Nutr., 37, 693 (1997)

[23] Stoner DG, Mukhtar H, J. Cell. Biochem., 22, 169 (1995)

[24] Yang SC, Maliakal P, Meng X, Annu. Rev. of Pharmacol. Toxicol., 42, 25 (2002)

[25] Su YL, Leung LK, Huang Y, Chen ZY, Food Chem., 83, 189 (2003)

[26] Proniuk S, Liederer BM, Blanchard J, J. Pharmacol. Sci., 91, 111 (2002)

[27] Wang X, Quinn PJ, Prog. Lipid Res., 38, 309 (1999)

[28] Di Mambro VM, Azzolini AECS, Valim YML, Fonseca MJV, Int. J. Pharm., 262, 99 (2003)