Synthetic dyes are released into the environment from textile industrial effluents. The discharge of this colored wastewater into rivers and lakes leads to a reduction in sunlight penetration in natural water bodies, which, in turn, decreases both photosynthetic activity and dissolved oxygen concentration and is toxic to living beings. Bacterial isolates are optimized for growth and biomass production before using them for decolorizing dye effluent. The bacterial isolates Bacillus sp. 1 and Bacillus sp. 2 were employed at different percentages by volume with standard nutrient concentration. Of these bacterial isolates Bacillus sp. 2 recorded maximum color reduction. The pH and electrical conductivity (EC) were reduced in the decolorized effluent, and a reduction in biologic oxygen demand, chemical oxygen demand, total suspended solids, and total dissolved solids (TDS) were also observed.