An efficient system for the fast and efficient purification of transglutaminase from recombinant Streptomyces platensis and expressed in Streptomyces lividans 25-2 is described. Because the purification procedure of this system is flexible, culture broth from laboratory (20 1) and pilot-plant (130 1) fermentations were used to purify the enzyme to electrophoretic homogeneity with high purity (90-95%) and yield (61-77%) within 1 or 2 days.