Application of femtosecond laser ablation for detaching grown protein crystals from glass capillary tube

Kashii M; Fujisawa R; Adachi H; Mori Y; Sasaki T; Koga Y; Takano K; Kanaya S; Matsumura H; Inoue T; Murakami S; Sugamoto K; Yoshikawa H

Journal of Bioscience and Bioengineering, Vol.102, No.4, 372-374, 2006

Kashii M, Fujisawa R, Adachi H, Mori Y, Sasaki T, Koga Y, Takano K, Kanaya S, Matsumura H, Inoue T, Murakami S, Sugamoto K, Yoshikawa H

We developed a novel technique for detaching protein crystals from glass capillary tube using the counter diffusion crystallization technique by femtosecond laser irradiation. X-ray diffraction analysis demonstrated that femtosecond laser irradiation has little effect on crystallinity. This technique will contribute to progress in structural genomics as a powerful tool.

Keywords:femtosecond laser;laser ablation;gel tube;counter diffusion technique;protein crystal;X-ray diffraction