In this study, the kinetic behaviors between n- butyl acetate and composite bead were investigated. Both microbial growth rate and biochemical reaction rate would be inhibited with increasing average inlet concentration. The order of the inhibitive effect, which resulted from increased average inlet concentration for four operation temperatures, was 30 > 35 > 40 > 25 degrees C. Both microbial growth rate and biochemical reaction rate would be enhanced and inhibited with increasing operation temperature in the operation temperature ranges of 25 to 30 and 30 to 40 degrees C, respectively. The enhancing and inhibitive effects resulting from increased operation temperature were the most pronounced at the average inlet concentration of 200 ppm. Thevalues of maximum reaction rate V-m and half-saturation constant K-s ranged from 0.011 to 0.047 g C h(-1) kg(-1) packed material and from 19.30 to 62.40 ppm, respectively. The zero-order kinetic with the diffusion rate limitation could be regarded as the most adequate biochemical reaction kinetic model. The values of maximum elimination capacity ranged from 0.51 to 0.20 g C h(-1) kg(-1) packed material, and the optimal maximum elimination capacity of biofilter occurred at the operation temperature of 30 degrees C.