Journal of Applied Polymer Science, Vol.102, No.2, 1341-1355, 2006
Adsorption of fibrinogen onto macroporous, biocompatible sponges based on poly(2-hydroxyethyl methacrylate)
Hydrophilic, spongy matrices of poly (2-hydroxyethyl methacrylate) were synthesized by a redox polymerization method, and the adsorption of fibrinogen was carried out on their surfaces. The prepared sponges were characterized with Fourier transform infrared and environmental scanning electron microscopy and were assayed for their water-sorption potential. The chemical architecture of the sponges had a pronounced impact on both the water sorption and the protein adsorption affinity of the sponge surface. The adsorption kinetics were investigated, and kinetic parameters such as the rate constants for adsorption and desorption and the penetrate rate constant were evaluated. The influence of experimental conditions such as the pH and temperature were observed on the adsorption profiles of fibrinogen. The prepared sponges were also judged for in vitro blood compatibility by methods such as bloodclot-formation and hemolysis-percentage tests. An attempt was also made to correlate the fibrinogen adsorption capacity of the sponge to its antithrombogenic response to static blood. (c) 2006 Wiley Periodicals, Inc.