This paper describes a facile, reproducible soft-lithography-based method for fabricating hexagonally close-packed microlens arrays by templating the surface of a colloidal monolayer, which is formed by spin-casting monodisperse polystyrene microspheres. The relief structure of colloidal monolayers has successfully generated PDMS elastomers with hexagonal arrays of hemispherical air voids. Closely packed hemispherical microlens arrays were imprinted on ultraviolet-curable photopolymers which are bound on glass substrates. Atomic force microscopy measurements showed that each spherical hole of the PDMS molds is 103 nm deep and the replicated microlens is 95 nm in height with narrow size distribution and good reproducibility. Without a multistep engineering process, this method might provide a reliable route to fabricate embossed thin layers ranging from nanometer to micrometers by controlling the size of polymer microspheres over a centimeter scale area.